Abstract: The Ecological Quality Index (EQI) and Human Activity Intensity Index (HAI) was established by using a 1-km high-precision grid resolution to analyze their temporal and spatial changes and interrelationships through the four-quadrant method. The results reveal that over the recent 20 years in Fujian Province, the HAI has steadily increased, while the EQI initially declined before rising. High HAI values are predominantly found in densely populated coastal cities, whereas high EQI values are mainly located in inland mountainous areas. Most urban areas and roads fall within the high HAI and low EQI range, while forested mountainous regions are characterized by low HAI and high EQI. Over the past 20 years, the proportion of regions with both high HAI and EQI has increased by 3%. Areas experiencing both coordinated and conflicting changes each account for 9%. Conflicting changes are primarily observed in densely populated urban areas, while coordinated changes are mainly found in the outskirts of urban areas, with some scattered within the cities. Comparing the last 10 years with the previous decade, there has been a 4% increase in coordinated change areas and a 5% decrease in conflicting change areas. This shift indicates the effectiveness of ecological protection and governance measures implemented in Fujian Province over the past decade. These results demonstrate the regional coordinated development between human activities and the ecological environment in Fujian Province.